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Verwendung der wirbellosen Galleria mellonella als Infektionsmodell zur Untersuchung des Mycobacterium tuberculosis Complex
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Use of the Invertebrate Galleria mellonella as an Infection Model to Study the Mycobacterium tuberculosis Complex
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Verwendung der wirbellosen Galleria mellonella als Infektionsmodell zur Untersuchung des Mycobacterium tuberculosis Complex

11,520 Views

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09:23 min

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June 30, 2019

DOI:

10.3791/59703-v

DOI:
10.3791/59703-v

•

09:23 min
June 30, 2019

•

12 Views
, , , , , ,
1Section of Pediatric Infectious Diseases and Allergy, Department of Medicine,Imperial College London, 2Department of Pharmacy,National University of Singapore, 3MRC Centre for Molecular Bacteriology and Infection, Department of Medicine,Imperial College London

Summary

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Galleria mellonella wurde vor kurzem als reproduzierbares, billiges und ethisch akzeptables Infektionsmodell für den Mycobacterium tuberculosis-Komplex etabliert. Hier beschreiben und zeigen wir die Schritte, die unternommen wurden, um eine erfolgreiche Infektion von G. mellonella mit biolumineszierendem Mycobacterium bovis BCG lux zu etablieren.

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Cite this Article

Asai, M., Li, Y., Khara, J. S., Gladstone, C. A., Robertson, B. D., Langford, P. R., Newton, S. M. Use of the Invertebrate Galleria mellonella as an Infection Model to Study the Mycobacterium tuberculosis Complex. J. Vis. Exp. (148), e59703, doi:10.3791/59703 (2019).

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