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体外ELISA测试评估狂犬病疫苗效力
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
In Vitro ELISA Test to Evaluate Rabies Vaccine Potency
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体外ELISA测试评估狂犬病疫苗效力

DOI:
10.3791/59641-v

•

09:04 min

•

May 11, 2020

•

,
1Unit Antiviral Strategies, OIE Reference Laboratories for Rift Valley Fever Virus & Crimean Congo Hemorrhagic Fever Virus,Institut Pasteur, 2Institut Pasteur de Guinée, Conakry

Capítulos

  • 00:04Introduction
  • 01:08Microplate Sensitization
  • 02:07Microplate Passivation
  • 02:33ELISA Assay
  • 06:44Results: Analysis of the ELISA Test
  • 08:00Conclusion

Summary

Tadução automática

Tadução automática

在这里，我们描述了一个间接的ELISA三明治免疫捕获，以确定狂犬病疫苗中的免疫原性糖蛋白含量。本测试使用中和单克隆抗体（mAb-D1）识别糖蛋白修剪器。它是体内NIH测试的替代方案，用于在生产过程中遵循疫苗效力的一致性。

Tags

Keywords: ELISARabies VaccinePotencyIn VitroAnimal TestingNH TestImmunogenic GlycoproteinSensitivityIn VivoManufacturersNational Control LaboratoriesPersonal Protective EquipmentDecontaminationMonoclonal AntibodyPassivation BufferWashing BufferReference VaccineDilution

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