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微 Rna 对原生脂蛋白颗粒的富集及其与绝对相对微 Rna 含量及细胞转移速率的测定
JoVE Journal
Bioquímica
This content is Free Access.
JoVE Journal Bioquímica
Enrichment of Native Lipoprotein Particles with microRNA and Subsequent Determination of Their Absolute/Relative microRNA Content and Their Cellular Transfer Rate
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微 Rna 对原生脂蛋白颗粒的富集及其与绝对相对微 Rna 含量及细胞转移速率的测定

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11:13 min

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May 09, 2019

DOI:

10.3791/59573-v

DOI:
10.3791/59573-v

•

11:13 min
May 09, 2019

•

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1Center for Pathobiochemistry and Genetics, Institute of Medical Chemistry and Pathobiochemistry,Medical University Vienna, 2School of Medical Engineering and Applied Social Sciences,University of Applied Sciences Upper Austria

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Lipoprotein ParticlesMicroRNACellular UptakeDelipidationEnrichmentRelipidationBradford Protein AssayReconstitutionSpermineSynthetic MicroRNA

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Axmann, M., Karner, A., Meier, S. M., Stangl, H., Plochberger, B. Enrichment of Native Lipoprotein Particles with microRNA and Subsequent Determination of Their Absolute/Relative microRNA Content and Their Cellular Transfer Rate. J. Vis. Exp. (147), e59573, doi:10.3791/59573 (2019).

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