É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
Chromatin Immunoprecipitation Assay Using Micrococcal Nucleases in Mammalian Cells
13,546 Views
•
11:42 min
•
May 10, 2019
DOI:
Summary
Automatically generated
Chromatin immunoprecipitation (ChIP) is a powerful tool for understanding the molecular mechanisms of gene regulation. However, the method involves difficulties in obtaining reproducible chromatin fragmentation by mechanical shearing. Here, we provide an improved protocol for a ChIP assay using enzymatic digestion.
Read Article
Cite this Article
Yamakawa, T., Itakura, K. Chromatin Immunoprecipitation Assay Using Micrococcal Nucleases in Mammalian Cells. J. Vis. Exp. (147), e59375, doi:10.3791/59375 (2019).
Copy