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Chromatin Immunoprecipitation Assay Using Micrococcal Nucleases in Mammalian Cells
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11:42 min
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May 10, 2019
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Chromatin immunoprecipitation (ChIP) is a powerful tool for understanding the molecular mechanisms of gene regulation. However, the method involves difficulties in obtaining reproducible chromatin fragmentation by mechanical shearing. Here, we provide an improved protocol for a ChIP assay using enzymatic digestion.
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Yamakawa, T., Itakura, K. Chromatin Immunoprecipitation Assay Using Micrococcal Nucleases in Mammalian Cells. J. Vis. Exp. (147), e59375, doi:10.3791/59375 (2019).
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