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Chromatin Immunoprecipitation Assay Using Micrococcal Nucleases in Mammalian Cells
JoVE Journal
Genetik
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JoVE Journal Genetik
Chromatin Immunoprecipitation Assay Using Micrococcal Nucleases in Mammalian Cells

Chromatin Immunoprecipitation Assay Using Micrococcal Nucleases in Mammalian Cells

13,601 Views

11:42 min

May 10, 2019

DOI:

11:42 min
May 10, 2019

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Summary

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Chromatin immunoprecipitation (ChIP) is a powerful tool for understanding the molecular mechanisms of gene regulation. However, the method involves difficulties in obtaining reproducible chromatin fragmentation by mechanical shearing. Here, we provide an improved protocol for a ChIP assay using enzymatic digestion.

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