Journal
/
Imunologia e Infecção
/
Co-staining Blood Vessels and Nerve Fibers in Adipose Tissue
JoVE Journal
Imunologia e Infecção
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Imunologia e Infecção
Co-staining Blood Vessels and Nerve Fibers in Adipose Tissue

Co-staining Blood Vessels and Nerve Fibers in Adipose Tissue

8,790 Views

•

12:05 min

•

February 13, 2019

DOI:

10.3791/59266-v

DOI:
10.3791/59266-v

•

12:05 min
February 13, 2019

•

1 Views
, , ,
1Center for Metabolic and Degenerative Diseases, the Brown Foundation of Institute of Molecular Medicine,University of Texas Health Science Center at Houston, 2Microscopy Core, the Brown Foundation of Institute of Molecular Medicine,University of Texas Health Science Center at Houston

Summary

Automatically generated

New blood vessel formation and sympathetic innervation play pivotal roles in adipose tissue remodeling. However, there remain technical issues in visualizing and quantitatively measuring adipose tissue. Here we present a protocol to successfully label and quantitatively compare the densities of blood vessels and nerve fibers in different adipose tissues.

Explore More Videos

AngiogenesisSympathetic InnervationBlood VesselsNerve FibersImmunofluorescenceConfocal MicroscopyFixationPermeabilizationBlockingPrimary AntibodiesEndomucinTyrosine Hydroxylase

Read Article

Cite this Article

Li, X., Mao, Z., Yang, L., Sun, K. Co-staining Blood Vessels and Nerve Fibers in Adipose Tissue. J. Vis. Exp. (144), e59266, doi:10.3791/59266 (2019).

Download .ris file

Copy

Share Video

.

Copy