Journal
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Immunologie et infection
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Co-staining Blood Vessels and Nerve Fibers in Adipose Tissue
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Immunologie et infection
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Journal JoVE Immunologie et infection
Co-staining Blood Vessels and Nerve Fibers in Adipose Tissue

Co-staining Blood Vessels and Nerve Fibers in Adipose Tissue

8,790 Views

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12:05 min

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February 13, 2019

DOI:

10.3791/59266-v

DOI:
10.3791/59266-v

•

12:05 min
February 13, 2019

•

1 Views
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1Center for Metabolic and Degenerative Diseases, the Brown Foundation of Institute of Molecular Medicine,University of Texas Health Science Center at Houston, 2Microscopy Core, the Brown Foundation of Institute of Molecular Medicine,University of Texas Health Science Center at Houston

Summary

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New blood vessel formation and sympathetic innervation play pivotal roles in adipose tissue remodeling. However, there remain technical issues in visualizing and quantitatively measuring adipose tissue. Here we present a protocol to successfully label and quantitatively compare the densities of blood vessels and nerve fibers in different adipose tissues.

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Cite this Article

Li, X., Mao, Z., Yang, L., Sun, K. Co-staining Blood Vessels and Nerve Fibers in Adipose Tissue. J. Vis. Exp. (144), e59266, doi:10.3791/59266 (2019).

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