このコンテンツを視聴するには、JoVE 購読が必要です。 サインイン又は無料トライアルを申し込む。
Co-staining Blood Vessels and Nerve Fibers in Adipose Tissue
章
- 00:04標題
- 00:53Adipose Tissue Collection and Antibody Incubation
- 05:17Image Acquisition and Analysis of Blood Vessel and Nerve Fiber Networks
- 09:37Results: Whole Mount Co-immunofluorescence Staining of Blood Vessels and Nerve Fibers in Adipose Tissues
- 11:17Conclusion
New blood vessel formation and sympathetic innervation play pivotal roles in adipose tissue remodeling. However, there remain technical issues in visualizing and quantitatively measuring adipose tissue. Here we present a protocol to successfully label and quantitatively compare the densities of blood vessels and nerve fibers in different adipose tissues.
