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Preparation of Fungal and Plant Materials for Structural Elucidation Using Dynamic Nuclear Polarization Solid-State NMR
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JoVE Journal Bioquímica
Preparation of Fungal and Plant Materials for Structural Elucidation Using Dynamic Nuclear Polarization Solid-State NMR

Preparation of Fungal and Plant Materials for Structural Elucidation Using Dynamic Nuclear Polarization Solid-State NMR

7,299 Views

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09:37 min

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February 12, 2019

DOI:

10.3791/59152-v

DOI:
10.3791/59152-v

•

09:37 min
February 12, 2019

•

8 Views
, , , , ,
1Department of Chemistry,Louisiana State University, 2National High Magnetic Field Laboratory, 3Departments of Pediatrics, and Microbiology, Immunology and Parasitology,Louisiana State University Health Sciences Center

Summary

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A protocol for preparing 13C,15N-labeled fungal and plant samples for multidimensional solid-state NMR spectroscopy and dynamic nuclear polarization (DNP) investigations is presented.

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Cite this Article

Kirui, A., Dickwella Widanage, M. C., Mentink-Vigier, F., Wang, P., Kang, X., Wang, T. Preparation of Fungal and Plant Materials for Structural Elucidation Using Dynamic Nuclear Polarization Solid-State NMR. J. Vis. Exp. (144), e59152, doi:10.3791/59152 (2019).

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