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Förster Resonance Energy Transfer Measurements in Living Plant Cells
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JoVE Journal Bioquímica
Förster Resonance Energy Transfer Measurements in Living Plant Cells

Förster Resonance Energy Transfer Measurements in Living Plant Cells

DOI:
10.3791/62758-v

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06:53 min

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June 28, 2021

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1Dynamic Cell Imaging, Biochemistry and Physiology of Plants, Faculty of Biology,Bielefeld University

Capítulos

  • 00:04Introduction
  • 00:25Laser Adjustment
  • 02:03Adjustment of Photomultipliers
  • 02:57Frster Resonance Energy Transfer (FRET) Image Acquisition
  • 04:09Determination of Crosstalk Corrections, Calibration of the Measurements, and Data Evaluation
  • 05:13Results: FRET Measurements in Living Plant Cells
  • 06:22Conclusion

Summary

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A protocol is provided for setting up a standard confocal laser-scanning microscope for in vivo Förster resonance energy transfer measurements, followed by data evaluation.

Tags

FRETFörster Resonance Energy TransferProtoplastsLaser AdjustmentPhotomultiplierReflection FilterLine ScanningHistogram ViewDetector GainLaser IntensityData EvaluationImage AcquisitionWater Immersion ObjectiveLive Cells

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