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Large-Scale Multi-Omics Genome-Wide Association Studies (Mo-GWAS): Guidelines for Sample Preparation and Normalization
JoVE Journal
Bioquímica
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JoVE Journal Bioquímica
Large-Scale Multi-Omics Genome-Wide Association Studies (Mo-GWAS): Guidelines for Sample Preparation and Normalization

Large-Scale Multi-Omics Genome-Wide Association Studies (Mo-GWAS): Guidelines for Sample Preparation and Normalization

DOI:
10.3791/62732-v

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08:27 min

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July 27, 2021

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, ,
1Max-Planck-Institute of Molecular Plant Physiology, 2Center of Plant Systems Biology and Biotechnology

Capítulos

  • 00:04Introduction
  • 00:48Preparation of Biological Plant Material
  • 01:57Sample Extraction
  • 03:35UHPLC-MS Analysis of Semi-Polar Metabolites and Normalization of Large-Scale Metabolomics Dataset
  • 05:37Genome-Wide Association Studies (GWAS)
  • 06:26Results: Metabolomics Data Normalization and QTL Visualization
  • 07:45Conclusion

Summary

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Tadução automática

In this protocol, we present an optimized workflow, which combines an efficient and fast sample preparation of many samples. In addition, we provide a step-by-step guide to reduce analytical variations for high-throughput evaluation of metabolic GWAS studies.

Tags

Multi-omicsGenome-wide Association StudiesMo-GWASSample PreparationNormalizationTechnical VariationBiological VarianceMetabolitesAnalytical PlatformGenotypic InformationPhenotypic InformationNatural PopulationFlash FreezingExtractionCentrifugationSolvent EvaporationGCMSUHPLC-MS

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