/

/

微胶质过程对急性脑片 atp 或血清素的双光子成像

JoVE Journal
Neurociência

É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.

JoVE Journal Neurociência

Two-photon Imaging of Microglial Processes’ Attraction Toward ATP or Serotonin in Acute Brain Slices

Please note that all translations are automatically generated. Click here for the English version.

微胶质过程对急性脑片 atp 或血清素的双光子成像

DOI:

10.3791/58788-v

•

07:27 min

•

January 31, 2019

•

Fanny Etienne^2,3, Vincenzo Mastrolia^2,3, Luc Maroteaux^2,3, Jean-Antoine Girault^2,3, Nicolas Gervasi*^1,2,3, Anne Roumier*^1,2,3

¹Institut National de la Santé et de la Recherche Médicale (INSERM), UMR-S 1270 Paris, France, ²Sorbonne Université, Paris, France, ³Institut du Fer à Moulin, Paris, France

Capítulos

00:04Título
00:42Dissection Area Preparation
01:51Brain Slicing
03:112-photon Live Imaging
04:12Compound Application
06:14Results: Microglial Response Analyses
07:05Conclusion

Summary

Tadução automática

小胶质细胞是大脑中的常驻免疫细胞, 随着环境变化的形态变化而迅速反应。该协议描述了如何使用双光子显微镜研究微胶质过程对血清素或 atp 的吸引力, 在急性脑切片的小鼠。

Tags

Keywords: Two-photon Imaging Microglial Processes ATP Serotonin Acute Brain Slices Directional Motility 3D Print Interface Perfusion Chambers Choline ACSF Vibrating Slicer Interface Chamber Peristaltic Pump Recording Chamber

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

钙成像的急性下区片的制备

在体内双光子成像的经验依赖的分子在大脑皮质神经元的变化

成神经细胞迁移的小鼠急性脑片在体内电穿孔产后和时间推移成像

细胞动力学在小鼠脊髓双光子成像

用优化的n-甲基 d-葡乙胺保护恢复法制备急性脑切片

小容积回收-灌注-浸没式室系统中维持的急性海马切片的突触可塑性记录

脑切片神经元树突的双光子钙成像

体内新生小鼠皮层神经元的双光子成像

使用基于 FRET 的传感器 ATeam1.03^YEMK在小鼠大脑的组织切片中进行细胞内 ATP 成像

小鼠视网膜的经瞳双光子体内成像

Read Article