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用奇米蛋白构建功能蛋白区的鉴定
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Bioquímica
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JoVE Journal Bioquímica
Identification of Functional Protein Regions Through Chimeric Protein Construction
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用奇米蛋白构建功能蛋白区的鉴定

10,179 Views

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11:39 min

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January 08, 2019

DOI:

10.3791/58786-v

DOI:
10.3791/58786-v

•

11:39 min
January 08, 2019

•

9 Views
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1Department of Cardiac Development and Remodelling,Max Planck Institute for Heart and Lung Research, 2Partner site Rhein-Main,German Centre for Cardiovascular Research (DZHK)

Summary

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结构上相关的蛋白质经常发挥不同的生物学功能。交换这些蛋白质的等效区域, 以创造嵌合蛋白是一种创新的方法, 以确定关键的蛋白质区域, 负责其功能分化。

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Cite this Article

Adrian-Segarra, J. M., Lörchner, H., Braun, T., Pöling, J. Identification of Functional Protein Regions Through Chimeric Protein Construction. J. Vis. Exp. (143), e58786, doi:10.3791/58786 (2019).

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