É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
1Ming Wai Lau Centre for Reparative Medicine,Karolinska Institutet, 2Department of Chemistry, Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and Reconstruction, Institute of Molecular Functional Materials, State Key Laboratory of Neuroscience, Division of Biomedical Engineering, and Division of Life Science,Hong Kong University of Science and Technology
Capítulos
- 00:04Título
- 00:36Preparation of the Gel
- 01:08Fixation of the Gel
- 01:40Preparation of the AgNO3 Solution and Silver Impregnation of the Gel
- 02:41Fluorogenic Development of the Gel
- 04:05Destaining and Imaging
- 04:34Results: Comparison of the Fluorescent Silver Staining to Other Staining Methods
- 05:56Conclusion
Her beskriver vi en detaljert protokoll beskriver en ny fluorescerende flekker teknikk for totalt proteinet oppdagelsen i polyakrylamid gels. Protokollen bruker en sølv ion-spesifikke fluorescens turn-on sonde, som oppdager Ag+-protein komplekser, og eliminerer visse begrensninger av tradisjonelle kromogent sølv flekker.
