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Fluorescent Silver Staining of Proteins in Polyacrylamide Gels
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06:24 min
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April 21, 2019
DOI:
1Ming Wai Lau Centre for Reparative Medicine,Karolinska Institutet, 2Department of Chemistry, Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and Reconstruction, Institute of Molecular Functional Materials, State Key Laboratory of Neuroscience, Division of Biomedical Engineering, and Division of Life Science,Hong Kong University of Science and Technology
Summary
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Here, we describe a detailed protocol outlining a new fluorescent staining technique for total protein detection in polyacrylamide gels. The protocol utilizes a silver ion-specific fluorescence turn-on probe, which detects Ag+-protein complexes, and eliminates certain limitations of traditional chromogenic silver stains.
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Wong, A. Y. H., Xie, S., Tang, B. Z., Chen, S. Fluorescent Silver Staining of Proteins in Polyacrylamide Gels. J. Vis. Exp. (146), e58669, doi:10.3791/58669 (2019).
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