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Fluorescent Silver Staining of Proteins in Polyacrylamide Gels
JoVE 杂志
生物化学
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JoVE 杂志 生物化学
Fluorescent Silver Staining of Proteins in Polyacrylamide Gels

Fluorescent Silver Staining of Proteins in Polyacrylamide Gels

13,886 Views

06:24 min

April 21, 2019

DOI:

06:24 min
April 21, 2019

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Summary

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Here, we describe a detailed protocol outlining a new fluorescent staining technique for total protein detection in polyacrylamide gels. The protocol utilizes a silver ion-specific fluorescence turn-on probe, which detects Ag+-protein complexes, and eliminates certain limitations of traditional chromogenic silver stains.

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