Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich. Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
Fluorescent Silver Staining of Proteins in Polyacrylamide Gels
13,886 Views
•
06:24 min
•
April 21, 2019
DOI:
1Ming Wai Lau Centre for Reparative Medicine,Karolinska Institutet, 2Department of Chemistry, Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and Reconstruction, Institute of Molecular Functional Materials, State Key Laboratory of Neuroscience, Division of Biomedical Engineering, and Division of Life Science,Hong Kong University of Science and Technology
Summary
Automatically generated
Here, we describe a detailed protocol outlining a new fluorescent staining technique for total protein detection in polyacrylamide gels. The protocol utilizes a silver ion-specific fluorescence turn-on probe, which detects Ag+-protein complexes, and eliminates certain limitations of traditional chromogenic silver stains.
Read Article
Cite this Article
Wong, A. Y. H., Xie, S., Tang, B. Z., Chen, S. Fluorescent Silver Staining of Proteins in Polyacrylamide Gels. J. Vis. Exp. (146), e58669, doi:10.3791/58669 (2019).
Copy