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Erfassung und Identifizierung von RNA-bindende Proteine mit Click-Chemie-gestützte RNA-Interaktom erfassen (CARIC) Strategie

JoVE Journal
Bioquímica

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JoVE Journal Bioquímica

Capture and Identification of RNA-binding Proteins by Using Click Chemistry-assisted RNA-interactome Capture (CARIC) Strategy

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Erfassung und Identifizierung von RNA-bindende Proteine mit Click-Chemie-gestützte RNA-Interaktom erfassen (CARIC) Strategie

9,130 Views

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09:36 min

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October 19, 2018

DOI:

10.3791/58580-v

DOI:

10.3791/58580-v

•

09:36 min

October 19, 2018

•

4 Views

Rongbing Huang*^1,2, Mengting Han*^1,2, Liying Meng³, Xing Chen^2,3,4,5

¹College of Chemistry and Molecular Engineering,Peking University, ²Beijing National Laboratory for Molecular Sciences,Peking University, ³Peking-Tsinghua Center for Life Sciences,Peking University, ⁴Synthetic and Functional Biomolecules Center,Peking University, ⁵Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education,Peking University

Tags

RNA-binding Proteins CARIC Click Chemistry RNA-interactome Capture Post-transcriptional Gene Regulation MRNA Non-coding RNA HeLa Cells EU 4SU UV Cross-linking Cell Lysis RNA-protein Interaction

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