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种系线虫与胸苷模拟教育的细胞周期分析
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Cell Cycle Analysis in the C. elegans Germline with the Thymidine Analog EdU
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种系线虫与胸苷模拟教育的细胞周期分析

11,062 Views

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06:58 min

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October 22, 2018

DOI:

10.3791/58339-v

DOI:
10.3791/58339-v

•

06:58 min
October 22, 2018

•

1 Views
, , ,
1Department of Developmental Biology,Washington University School of Medicine, St. Louis, Missouri, 2Department of Genetics,Washington University School of Medicine, St. Louis, Missouri

Tags

Cell Cycle AnalysisC. ElegansGermlineEdUThymidine AnalogImmunofluorescent StainingGerm Line Stem CellCell Cycle DynamicsAgingNutritionAltered GenotypesIdiodisprepationHematoxylin StainingSterile TechniqueM9 BufferE. ColiEdU SupplementationCentrifugationNematode Growth MediumPBSDissectionFixation

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Kocsisova, Z., Mohammad, A., Kornfeld, K., Schedl, T. Cell Cycle Analysis in the C. elegans Germline with the Thymidine Analog EdU. J. Vis. Exp. (140), e58339, doi:10.3791/58339 (2018).

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