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虫媒病毒感染作为鉴定病毒免疫调节剂和宿主抗病毒因子的筛选工具
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Arbovirus Infections As Screening Tools for the Identification of Viral Immunomodulators and Host Antiviral Factors
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虫媒病毒感染作为鉴定病毒免疫调节剂和宿主抗病毒因子的筛选工具

DOI:
10.3791/58244-v

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06:02 min

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September 13, 2018

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, ,
1Department of Microbiology,University of Texas Southwestern Medical Center

Capítulos

  • 00:04Título
  • 00:48General Lymantria dispar (LD652) Cell and Virus Culture
  • 01:33General Viral Infection Protocol for Luminescence-based VSV rescue Assays in LD652 Cells
  • 02:43Luciferase Assay
  • 04:06Results: Coinfection with VSV-LUC with VACV-WR Results in More LUC Signals
  • 05:04Conclusion

Summary

Tadução automática

Tadução automática

在这里, 我们提出了识别 1) 病毒编码的免疫调节剂的协议, 以促进虫媒病毒复制和 2) 限制虫媒病毒复制的真核宿主因素。这些荧光和发光的方法, 使研究人员能够迅速获得定量读数的虫媒病毒复制在简单的检测, 低信噪比。

Tags

ArbovirusViral ImmunologyHost FactorsViral Immune EvasionLuminescence AssayFluorescent AssayLepidopteran Insect CellsVSV LuciferaseVaccinia VirusReporter Lysis Buffer

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