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Een High-throughput Cre-Lox geactiveerd virale membraan Fusion Assay ter identificatie van de Inhibitors van HIV-1 virale membraan Fusion
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
A High-throughput Cre-Lox Activated Viral Membrane Fusion Assay to Identify Inhibitors of HIV-1 Viral Membrane Fusion
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Een High-throughput Cre-Lox geactiveerd virale membraan Fusion Assay ter identificatie van de Inhibitors van HIV-1 virale membraan Fusion

6,225 Views

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07:22 min

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August 14, 2018

DOI:

10.3791/58074-v

DOI:
10.3791/58074-v

•

07:22 min
August 14, 2018

•

1 Views
, , , , ,
1Division of Infectious Diseases, Department of Medicine,Icahn School of Medicine at Mount Sinai, Immunology Institute, 2Department of Biology,New Jersey City University

Summary

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We beschrijven een cel-gebaseerde test om te melden aan de HIV-1 fusion via de uitdrukking van groen fluorescent proteïne aantoonbaar door stroom cytometry of fluorescentie microscopie. Het kan worden gebruikt voor het testen van remmers van virale toegang (specifiek aan de fusie stap) in cel-vrij en cel-naar-cel infectie systemen.

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Cite this Article

Esposito, A. M., Soare, A. Y., Patel, F., Satija, N., Chen, B. K., Swartz, T. H. A High-throughput Cre-Lox Activated Viral Membrane Fusion Assay to Identify Inhibitors of HIV-1 Viral Membrane Fusion. J. Vis. Exp. (138), e58074, doi:10.3791/58074 (2018).

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