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小鼠原代多元体的体外模拟？

JoVE Journal
Neurociência

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JoVE Journal Neurociência

Modeling Charcot-Marie-Tooth Disease In Vitro by Transfecting Mouse Primary Motoneurons

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小鼠原代多元体的体外模拟？

DOI:

10.3791/57988-v

•

07:43 min

•

January 07, 2019

•

Arnaud Jacquier², Valérie Risson, Laurent Schaeffer³

¹Institut NeuroMyoGène, CNRS UMR5310, INSERM U1217,Université Lyon, ²Unité Fonctionnelle de Neurogénétique Moléculaire,Hospices Civils de Lyon, ³Centre de Biotechnologie Cellulaire,Hospices Civils de Lyon

Capítulos

00:04Título
00:36Dissection
01:49Spinal Cord Cell Suspension
02:58Motoneuron Enrichment by Gradient Density
04:42Motoneuron Culture and Magnetofection
06:00Results: Confocal Images of Magnetofected Motoneurons
06:59Conclusion

Summary

Tadução automática

这项技术的目的是准备一种高度丰富的来自小鼠脊髓的原发运动神经元 (mns) 培养。为了评估引起 mn 疾病的突变的后果, 我们在这里描述了这些孤立的 m2 的隔离和它们通过磁光的转染。

Tags

Charcot-Marie-Tooth Disease In Vitro Modeling Primary Motor Neurons Transfection Neurobiology Motor Neuron Polarity Axon Guidance Axon Trafficking Neurodegenerative Mechanisms Enriched Motor Neuron Culture Knockdown Protein Spinal Cord Dissection Enzymatic Digestion Cell Suspension Density Gradient Centrifugation Motoneuron Enrichment

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