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JoVE Journal Neuroscience
Modeling Charcot-Marie-Tooth Disease In Vitro by Transfecting Mouse Primary Motoneurons
 

Modeling Charcot-Marie-Tooth Disease In Vitro by Transfecting Mouse Primary Motoneurons

Article DOI: 10.3791/57988-v • 07:43 min • January 7th, 2019
January 7th, 2019 •
1,2, 1, 1,3
1Institut NeuroMyoGène, CNRS UMR5310, INSERM U1217, Université Lyon, 2Unité Fonctionnelle de Neurogénétique Moléculaire, Hospices Civils de Lyon, 3Centre de Biotechnologie Cellulaire, Hospices Civils de Lyon

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The goal of this technique is to prepare a highly enriched culture of primary motoneurons (MNs) from murine spinal cord. To evaluate the consequences of mutations causing MN diseases, we describe here the isolation of these isolated MNs and their transfection by magnetofection.

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Keywords: Charcot-Marie-Tooth Disease In Vitro Modeling Primary Motor Neurons Transfection Neurobiology Motor Neuron Polarity Axon Guidance Axon Trafficking Neurodegenerative Mechanisms Enriched Motor Neuron Culture Knockdown Protein Spinal Cord Dissection Enzymatic Digestion Cell Suspension Density Gradient Centrifugation Motoneuron Enrichment
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