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Modeling Charcot-Marie-Tooth Disease In Vitro by Transfecting Mouse Primary Motoneurons
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January 07, 2019
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The goal of this technique is to prepare a highly enriched culture of primary motoneurons (MNs) from murine spinal cord. To evaluate the consequences of mutations causing MN diseases, we describe here the isolation of these isolated MNs and their transfection by magnetofection.
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Charcot-Marie-Tooth DiseaseIn Vitro ModelingPrimary Motor NeuronsTransfectionNeurobiologyMotor Neuron PolarityAxon GuidanceAxon TraffickingNeurodegenerative MechanismsEnriched Motor Neuron CultureKnockdown ProteinSpinal Cord DissectionEnzymatic DigestionCell SuspensionDensity Gradient CentrifugationMotoneuron Enrichment
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Jacquier, A., Risson, V., Schaeffer, L. Modeling Charcot-Marie-Tooth Disease In Vitro by Transfecting Mouse Primary Motoneurons. J. Vis. Exp. (143), e57988, doi:10.3791/57988 (2019).
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