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Imunologia e Infecção
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Quantifying Vibrio cholerae Colonization and Diarrhea in the Adult Zebrafish Model
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Quantifying Vibrio cholerae Colonization and Diarrhea in the Adult Zebrafish Model

Quantifying Vibrio cholerae Colonization and Diarrhea in the Adult Zebrafish Model

DOI:
10.3791/57767-v

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08:03 min

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July 12, 2018

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, , ,
1Department of Microbiology, Immunology, and Biochemistry,Wayne State University School of Medicine

Capítulos

  • 00:04Título
  • 00:40Inoculating Zebrafish with V. cholerae
  • 01:49Collecting Intestines and Assessing Bacterial Colonization
  • 04:27Metrics for Fish Diarrhea: Mucin Levels, Protein Levels, A600 and Excreted Bacteria
  • 06:43Results: Colonization and Diarrhea, 18 h After a 6 h Infection Period
  • 07:35Conclusion

Summary

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Zebrafish are a natural Vibrio cholerae host and can be used to recapitulate and study the entire infectious cycle from colonization to transmission. Here, we demonstrate how to assess V. cholerae colonization levels and quantify diarrhea in zebrafish.

Tags

Vibrio CholeraeZebrafish ModelColonizationDiarrheaIntestinal MicrobiotaLife CycleInfectionDiagnosticDissectionIntestinal Tract

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