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Quantifying Vibrio cholerae Colonization and Diarrhea in the Adult Zebrafish Model
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JoVE Journal 免疫学と感染
Quantifying Vibrio cholerae Colonization and Diarrhea in the Adult Zebrafish Model

Quantifying Vibrio cholerae Colonization and Diarrhea in the Adult Zebrafish Model

DOI:
10.3791/57767-v

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08:03 min

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July 12, 2018

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, , ,
1Department of Microbiology, Immunology, and Biochemistry,Wayne State University医学部

章

  • 00:04標題
  • 00:40Inoculating Zebrafish with V. cholerae
  • 01:49Collecting Intestines and Assessing Bacterial Colonization
  • 04:27Metrics for Fish Diarrhea: Mucin Levels, Protein Levels, A600 and Excreted Bacteria
  • 06:43Results: Colonization and Diarrhea, 18 h After a 6 h Infection Period
  • 07:35Conclusion

概要

自動翻訳

自動翻訳

Zebrafish are a natural Vibrio cholerae host and can be used to recapitulate and study the entire infectious cycle from colonization to transmission. Here, we demonstrate how to assess V. cholerae colonization levels and quantify diarrhea in zebrafish.

タグ

Vibrio CholeraeZebrafish ModelColonizationDiarrheaIntestinal MicrobiotaLife CycleInfectionDiagnosticDissectionIntestinal Tract

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