Journal
/
Inmunología y contagio
/
Quantifying Vibrio cholerae Colonization and Diarrhea in the Adult Zebrafish Model
JoVE Revista
Inmunología y contagio
Se requiere una suscripción a JoVE para ver este contenido.  Inicie sesión o comience su prueba gratuita.
JoVE Revista Inmunología y contagio
Quantifying Vibrio cholerae Colonization and Diarrhea in the Adult Zebrafish Model

Quantifying Vibrio cholerae Colonization and Diarrhea in the Adult Zebrafish Model

DOI:
10.3791/57767-v

•

08:03 min

•

July 12, 2018

•

, , ,
1Department of Microbiology, Immunology, and Biochemistry,Wayne State University School of Medicine

Capítulos

  • 00:04Título
  • 00:40Inoculating Zebrafish with V. cholerae
  • 01:49Collecting Intestines and Assessing Bacterial Colonization
  • 04:27Metrics for Fish Diarrhea: Mucin Levels, Protein Levels, A600 and Excreted Bacteria
  • 06:43Results: Colonization and Diarrhea, 18 h After a 6 h Infection Period
  • 07:35Conclusion

Summary

Traducción Automática

Traducción Automática

Zebrafish are a natural Vibrio cholerae host and can be used to recapitulate and study the entire infectious cycle from colonization to transmission. Here, we demonstrate how to assess V. cholerae colonization levels and quantify diarrhea in zebrafish.

Tags

Vibrio CholeraeZebrafish ModelColonizationDiarrheaIntestinal MicrobiotaLife CycleInfectionDiagnosticDissectionIntestinal Tract

Article

Embed

Añadir a la lista de reproducción

Usage Statistics

Videos relacionados

Assessing Hepatic Metabolic Changes During Progressive Colonization of Germ-free Mouse by 1H NMR Spectroscopy

Colonization of Euprymna scolopes Squid by Vibrio fischeri

TransFLP — A Method to Genetically Modify Vibrio cholerae Based on Natural Transformation and FLP-recombination

Culturing Microglia from the Neonatal and Adult Central Nervous System

Investigating the Effects of Probiotics on Pneumococcal Colonization Using an In Vitro Adherence Assay

Normal and Malignant Muscle Cell Transplantation into Immune Compromised Adult Zebrafish

Development of a More Sensitive and Specific Chromogenic Agar Medium for the Detection of Vibrio parahaemolyticus and Other Vibrio Species

A Murine Model of Group B Streptococcus Vaginal Colonization

Laboratory Techniques Used to Maintain and Differentiate Biotypes of Vibrio cholerae Clinical and Environmental Isolates

Quantifying Human Monocyte Chemotaxis In Vitro and Murine Lymphocyte Trafficking In Vivo

Read Article