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全挂载原位杂交法可视化蜱内脏微生物群
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JoVE Journal Genética
Visualization of Microbiota in Tick Guts by Whole-mount In Situ Hybridization
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全挂载原位杂交法可视化蜱内脏微生物群

DOI:
10.3791/57758-v

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08:30 min

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June 01, 2018

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, , ,
1Department of Microbial Pathogenesis,Yale University School of Medicine, 2Program in Vertebrate Developmental Biology, Departments of Pediatrics and Genetics,Yale University School of Medicine, 3Department of Internal Medicine,Yale University School of Medicine

Capítulos

  • 00:05Título
  • 00:45Tick Gut Collection and Fixation
  • 02:06Construction of Mesh Sample Baskets and 24-well Basket Holder
  • 03:37In Situ Hybridization
  • 06:25Results: Tips for Success
  • 08:05Conclusion

Summary

Tadução automática

Tadução automática

在这里, 我们提出一个协议, 以空间和世俗评估的存在, 在蜱内脏有活力的微生物, 使用改良的全芒原位杂交方法。

Tags

MicrobiotaTick GutsWhole-mount In Situ HybridizationTick EntomologyBacteriaGene TranscriptsMEMFA SolutionRazor BladesSalivary GlandsNylon Mesh24-well Plate

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