This protocol will enable readers to successfully establish a porcine model of segmental intestinal ischemia and subsequently isolate and culture intestinal stem cells for the study of epithelial repair following injury.
Stieler Stewart, A., Freund, J. M., Blikslager, A. T., Gonzalez, L. M. Intestinal Stem Cell Isolation and Culture in a Porcine Model of Segmental Small Intestinal Ischemia. J. Vis. Exp. (135), e57647, doi:10.3791/57647 (2018).