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Intestinal Stem Cell Isolation and Culture in a Porcine Model of Segmental Small Intestinal Ischemia
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医学
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JoVE Journal 医学
Intestinal Stem Cell Isolation and Culture in a Porcine Model of Segmental Small Intestinal Ischemia

Intestinal Stem Cell Isolation and Culture in a Porcine Model of Segmental Small Intestinal Ischemia

DOI:
10.3791/57647-v

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08:55 min

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May 18, 2018

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, , ,
1Department of Clinical Sciences,North Carolina State University

章

  • 00:04標題
  • 00:44Ischemia and Tissue Collection
  • 02:54Crypt Stem Cell Isolation
  • 06:12Results: Representative Crypt Isolation and Enteroid Generation
  • 07:48Conclusion

概要

自動翻訳

自動翻訳

This protocol will enable readers to successfully establish a porcine model of segmental intestinal ischemia and subsequently isolate and culture intestinal stem cells for the study of epithelial repair following injury.

タグ

Intestinal Stem CellsIschemiaPorcine ModelSmall IntestineCrypt IsolationEpithelial Stem CellsDissociation

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