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Intestinal Stem Cell Isolation and Culture in a Porcine Model of Segmental Small Intestinal Ischemia
JoVE Journal
Medizin
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JoVE Journal Medizin
Intestinal Stem Cell Isolation and Culture in a Porcine Model of Segmental Small Intestinal Ischemia

Intestinal Stem Cell Isolation and Culture in a Porcine Model of Segmental Small Intestinal Ischemia

DOI:
10.3791/57647-v

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08:55 min

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May 18, 2018

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, , ,
1Department of Clinical Sciences,North Carolina State University

Kapitel

  • 00:04Titel
  • 00:44Ischemia and Tissue Collection
  • 02:54Crypt Stem Cell Isolation
  • 06:12Results: Representative Crypt Isolation and Enteroid Generation
  • 07:48Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

This protocol will enable readers to successfully establish a porcine model of segmental intestinal ischemia and subsequently isolate and culture intestinal stem cells for the study of epithelial repair following injury.

Tags

Intestinal Stem CellsIschemiaPorcine ModelSmall IntestineCrypt IsolationEpithelial Stem CellsDissociation

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