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Canalostomy 为成人和新生小鼠内耳局部药物传递的外科治疗方法

JoVE Journal
Genética

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JoVE Journal Genética

Canalostomy As a Surgical Approach to Local Drug Delivery into the Inner Ears of Adult and Neonatal Mice

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Canalostomy 为成人和新生小鼠内耳局部药物传递的外科治疗方法

DOI:

10.3791/57351-v

•

09:34 min

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May 25, 2018

•

Jing-Ying Guo, Lu He, Teng-Fei Qu, Yu-Ying Liu, Ke Liu, Guo-Peng Wang, Shu-Sheng Gong

¹Department of Otolaryngology-Head and Neck Surgery, Beijing Friendship Hospital,Capital Medical University, ²Department of Otolaryngology-Head and Neck Surgery, Shanghai First People’s Hospital,Shanghai Jiao Tong University

Capítulos

00:05Título
00:56Device Preparation
02:31Canalostomy in Adult Mice
05:58Canalostomy in Neonatal Mice
06:48Results: Drug Delivery by Canalostomy Resulted in Widespread Distribution of the Reagent Throughout the Cochlea and Vestibular End-organs
08:13Conclusion

Summary

Tadução automática

在这里, 我们描述了 canalostomy 程序, 允许当地药物传递到成年和新生小鼠的内耳通过半圆形运河, 对听力和前庭功能的最小损害。该方法可用于将病毒载体、药物和小分子接种到小鼠内耳内。

Tags

Keywords: Canalostomy Inner Ear Drug Delivery Adult Mice Neonatal Mice Surgical Technique Polyimide Tubing Polyethylene Tubing Injection Cannula Fast Green Dye AAV8-GFP Streptomycin Microinjection Pump Postauricular Incision Semicircular Canal

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