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Canalostomy As a Surgical Approach to Local Drug Delivery into the Inner Ears of Adult and Neonatal Mice
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JoVE Revista Genética
Canalostomy As a Surgical Approach to Local Drug Delivery into the Inner Ears of Adult and Neonatal Mice

Canalostomy As a Surgical Approach to Local Drug Delivery into the Inner Ears of Adult and Neonatal Mice

10,215 Views

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09:34 min

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May 25, 2018

DOI:

10.3791/57351-v

DOI:
10.3791/57351-v

•

09:34 min
May 25, 2018

•

22 Views
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1Department of Otolaryngology-Head and Neck Surgery, Beijing Friendship Hospital,Capital Medical University, 2Department of Otolaryngology-Head and Neck Surgery, Shanghai First People’s Hospital,Shanghai Jiao Tong University

Summary

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Here we describe canalostomy procedure which allows local drug delivery into the inner ears of adult and neonatal mice through the semicircular canal with minimum damage to hearing and vestibular function. This method can be used to inoculate viral vectors, pharmaceuticals, and small molecules into the mouse inner ear.

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Cite this Article

Guo, J., He, L., Qu, T., Liu, Y., Liu, K., Wang, G., Gong, S. Canalostomy As a Surgical Approach to Local Drug Delivery into the Inner Ears of Adult and Neonatal Mice. J. Vis. Exp. (135), e57351, doi:10.3791/57351 (2018).

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