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Canalostomy As a Surgical Approach to Local Drug Delivery into the Inner Ears of Adult and Neonatal Mice
 

Canalostomy As a Surgical Approach to Local Drug Delivery into the Inner Ears of Adult and Neonatal Mice

Article DOI: 10.3791/57351-v • 09:34 min • May 25th, 2018
May 25th, 2018 •
1, 1, 1, 2, 1, 1, 1
1Department of Otolaryngology-Head and Neck Surgery, Beijing Friendship Hospital, Capital Medical University, 2Department of Otolaryngology-Head and Neck Surgery, Shanghai First People's Hospital, Shanghai Jiao Tong University

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Resumo
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Here we describe canalostomy procedure which allows local drug delivery into the inner ears of adult and neonatal mice through the semicircular canal with minimum damage to hearing and vestibular function. This method can be used to inoculate viral vectors, pharmaceuticals, and small molecules into the mouse inner ear.

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Canalostomy Surgical Approach Local Drug Delivery Inner Ears Adult Mice Neonatal Mice Surgical Techniques Injected Reagents Cochlear Vestibular End Organs Minimal Damage Hearing Function Vestibular Function Multiple Surgeries Polyimide Tubing Polyethylene Tubing Injection Cannula Super Glue Sterilize Ethylene Oxide One-CC Syringe Normal Saline Leakage Blockage Microsyringe Microinjection Pump Injection Speed Volume
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