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Journal / Neuroscience / 清醒小鼠海马毛细血管的体内纤维耦合临床前共聚焦激光扫描内窥镜…
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JoVE Journal Neuroscience
清醒小鼠海马毛细血管的体内纤维耦合临床前共聚焦激光扫描内窥镜 （pCLE）
 
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清醒小鼠海马毛细血管的体内纤维耦合临床前共聚焦激光扫描内窥镜 （pCLE）

Article DOI: 10.3791/57220-v • 09:08 min • April 21st, 2023
April 21st, 2023 •
1,2, 2,3, 2,3
1División de Neurociencias, Universidad Pablo de Olavide, 2Barrow Neurological Institute, 3Ophthalmology, Neurology, and Physiology/Pharmacology, SUNY Downstate Medical Center

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虽然多光子成像仅在距组织表面的有限深度有效，但可以通过pCLE在任何深度实现3μm分辨率成像。在这里，我们提出了一种进行pCLE成像的方案，以测量发作期和野生型小鼠海马体中的微血管动力学。

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纤维耦合临床前共聚焦激光扫描内窥镜、PCLE、海马毛细血管、清醒小鼠、毛细血管血流效应、癫痫发作、壁画细胞、体外皮质成像、周细胞、功能性局部神经活动、药物应用、健康动物、微血管动力学、神经变性、癫痫、组织深度、头部约束技术、清醒动物、麻醉剂、电生理记录、成像记录
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