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一种快速的银染色协议, 能够使用非变性聚丙烯酰胺凝胶对 SSR 标记进行简单而有效的检测
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JoVE Journal Genética
A Fast Silver Staining Protocol Enabling Simple and Efficient Detection of SSR Markers using a Non-denaturing Polyacrylamide Gel
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一种快速的银染色协议, 能够使用非变性聚丙烯酰胺凝胶对 SSR 标记进行简单而有效的检测

DOI:
10.3791/57192-v

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10:27 min

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April 20, 2018

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, , , , , ,
1College of Life Sciences,Guangzhou University, 2Hard Winter Wheat Genetics Research Unit,United States Department of Agriculture – Agricultural Research Service, 3The UWA Institute of Agriculture,University of Western Australia

Capítulos

  • 00:04Título
  • 01:38Preparation of Polyacrylamide Gels for Electrophoresis
  • 04:03Running Gels and Silver Staining for Detecting SSR Markers in a Non-polyacrylamide Gel
  • 07:43Results: Fast Silver Staining to Detect SSR Markers on a Non-denaturing Polyacrylamide Gel
  • 09:18Conclusion

Summary

Tadução automática

Tadução automática

在这里, 我们报告一个简单和低成本的银染色协议, 只需三试剂和7分钟的处理, 并适合快速生成高质量的 SSR 数据的遗传分析。

Tags

Silver StainingSSR MarkersNon-denaturing Polyacrylamide GelGenetic ResearchMarker-assisted SelectionMolecular BreedingRapid GenotypingEasyLow CostOptimized ProtocolBind-silaneRepel-silaneTEMEDAPSElectrophoresis

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