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Cell Membrane Repair Assay Using a Two-photon Laser Microscope
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Cell Membrane Repair Assay Using a Two-photon Laser Microscope

Cell Membrane Repair Assay Using a Two-photon Laser Microscope

11,916 Views

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06:35 min

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January 02, 2018

DOI:

10.3791/56999-v

DOI:
10.3791/56999-v

•

06:35 min
January 02, 2018

•

9 Views
, , ,
1Department of Medical Genetics,University of Alberta Faculty of Medicine and Dentistry, 2Center for iPS Cell Research and Application,Kyoto University

Summary

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Cell membrane wounding via two-photon laser is a widely used method for assessing membrane resealing ability and can be applied to multiple cell types. Here, we describe a protocol for in vitro live-imaging of membrane resealing in dysferlinopathy patient cells following two-photon laser ablation.

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Cite this Article

Lee, J. J. A., Maruyama, R., Sakurai, H., Yokota, T. Cell Membrane Repair Assay Using a Two-photon Laser Microscope. J. Vis. Exp. (131), e56999, doi:10.3791/56999 (2018).

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