/
/
Cell Membrane Repair Assay Using a Two-photon Laser Microscope
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich. Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
Cell Membrane Repair Assay Using a Two-photon Laser Microscope
Kapitel
- 00:05Titel
- 00:38Full-length Dysferlin (DYSF) Plasmid Cell Transfection
- 03:09Two-photon Laser Wounding Assay
- 05:19Results: Representative Fluorescence Quantification Over Time
- 06:00Conclusion
Cell membrane wounding via two-photon laser is a widely used method for assessing membrane resealing ability and can be applied to multiple cell types. Here, we describe a protocol for in vitro live-imaging of membrane resealing in dysferlinopathy patient cells following two-photon laser ablation.
