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脳スライスにおける神経樹状突起における 2 光子カルシウムイメージング

JoVE Journal
Neurociência

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JoVE Journal Neurociência

Two-photon Calcium Imaging in Neuronal Dendrites in Brain Slices

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脳スライスにおける神経樹状突起における 2 光子カルシウムイメージング

10,336 Views

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10:35 min

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March 15, 2018

DOI:

10.3791/56776-v

DOI:

10.3791/56776-v

•

10:35 min

March 15, 2018

•

12 Views

Olivier Camiré^1,2, Lisa Topolnik^1,2

¹CHU de Québec-Université Laval Research Center,Université Laval, ²Department of Biochemistry, Microbiology and Bioinformatics,Université Laval

Summary

全細胞のホールセルパッチクランプ記録と急性脳スライスにおける神経樹状突起における Ca^{2 +}過渡現象を記録する 2 光子イメージングを組み合わせた手法を提案します。

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