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Ved hjælp af en mikrofluidik anordning for mekanisk Stimulation og høj opløsning Imaging af C. elegans
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JoVE Journal Neurociência
Using a Microfluidics Device for Mechanical Stimulation and High Resolution Imaging of C. elegans

Ved hjælp af en mikrofluidik anordning for mekanisk Stimulation og høj opløsning Imaging af C. elegans

DOI:
10.3791/56530-v

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10:39 min

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February 19, 2018

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, , , , ,
1Department of Molecular and Cellular Physiology,Stanford University, 2Department of Mechanical Engineering,Stanford University, 3Department of Bioengineering,Stanford University, 4Group of Neurophotonics and Mechanical Systems Biology,The Institute of Photonic Sciences (ICFO)

Capítulos

  • 00:05Título
  • 00:52Preparation of the Microscope
  • 02:02Preparing the Microfluidic Chip
  • 03:45Loading the Test Subject
  • 06:02Stimulation and Removal of the Test Subject
  • 08:13Results: Touch Receptor Neuron (TRN) Stimuli
  • 09:42Conclusion

Summary

Tadução automática

Tadução automática

Nye værktøjer til mechanobiology forskning er nødvendig for at forstå hvordan mekanisk stress aktiveres biokemiske veje og fremkalder biologiske svar. Her, fremvise vi en ny metode til selektiv mekaniske stimulation af immobiliserede dyr med en mikrofluid fælde så høj opløsning billeddannelse af cellulære svar.

Tags

Keywords: MicrofluidicsMechanical StimulationHigh-resolution ImagingC. ElegansMechanobiologySensory BiologyPressure ActuatorsGCaMPRFPFluorescence MicroscopyM9 BufferPeristaltic PumpPDMS

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