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用小鼠模型对精子发生细胞学分析的精管壁球技术

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Biologia do Desenvolvimento

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JoVE Journal Biologia do Desenvolvimento

A Seminiferous Tubule Squash Technique for the Cytological Analysis of Spermatogenesis Using the Mouse Model

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用小鼠模型对精子发生细胞学分析的精管壁球技术

DOI:

10.3791/56453-v

•

09:40 min

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February 06, 2018

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Stephen R Wellard*¹, Jessica Hopkins*¹, Philip W. Jordan

¹Department of Biochemistry and Molecular Biology,Johns Hopkins Bloomberg School of Public Health

Capítulos

00:04Título
01:12Dissection and Preparation of Mouse Seminiferous Tubules
03:16Squashing and Labeling Prepared Seminiferous Tubules
05:47Analysis and Imaging Tubule Squash Preparations
07:03Results: Visualization of Meiotic Progression Using the Tubule Squash Preparation
08:35Conclusion

Summary

Tadução automática

这个小管壁技术的目的是快速评估发育中鼠精的细胞学特征, 同时保持细胞完整性。这种方法允许研究精子发生的所有阶段, 并可以很容易地实现与其他生物化学和分子生物学方法的研究小鼠减数分裂。

Tags

Keywords: Seminiferous Tubule Cytological Analysis Spermatogenesis Mouse Model Spermatogonia Spermatocytes Spermatids Meiosis Male Reproductive Biology Spermatogonial Stem Cell Differentiation Meiotic Chromosome Segregation Post-meiotic Differentiation Cellular Integrity Infertility Mammalian Systems Squash Technique PBS Fixative Lysis Solution Poly-L-lysine Coated Glass Slide

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