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Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein

Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein

9,137 Views

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07:29 min

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October 12, 2017

DOI:

10.3791/56325-v

DOI:
10.3791/56325-v

•

07:29 min
October 12, 2017

•

3 Views
, , , , , ,
1University of California, Los Angeles, 2Centre for Biomedical Research,Burnet Institute, 3School of Health and Biomedical Sciences,RMIT University, 4Department of Infectious Diseases,Monash University

Summary

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We describe here a fluorometric cell-free biochemical assay for determination of HDL-lipid peroxidation. This rapid and reproducible assay can be used to determine HDL function in large scale studies and can contribute to our understanding of HDL function in human disease.

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Cell-freeBiochemicalFluorometricEnzymatic AssayHigh-throughputLipid PeroxidationHigh Density LipoproteinHDL FunctionCardiovascular DiseaseMetabolic AbnormalitiesInflammatory DiseasesHDL-CHDLoxPooled Quality ControlHDL Cholesterol Precipitating ReagentHRPFluorochrome ReagentDMSO

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Sen Roy, S., Nguyen, H. C. X., Angelovich, T. A., Hearps, A. C., Huynh, D., Jaworowski, A., Kelesidis, T. Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein. J. Vis. Exp. (128), e56325, doi:10.3791/56325 (2017).

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