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Immunology and Infection

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JoVE Journal Immunology and Infection
Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein
 

Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein

Article DOI: 10.3791/56325-v • 07:29 min • October 12th, 2017
October 12th, 2017 •
1, 1, 2,3, 2, 1,4, 2,4, 1
1University of California, Los Angeles, 2Centre for Biomedical Research, Burnet Institute, 3School of Health and Biomedical Sciences, RMIT University, 4Department of Infectious Diseases, Monash University

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We describe here a fluorometric cell-free biochemical assay for determination of HDL-lipid peroxidation. This rapid and reproducible assay can be used to determine HDL function in large scale studies and can contribute to our understanding of HDL function in human disease.

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Cell-free Biochemical Assay Lipid Peroxidation High Density Lipoprotein HDL Function Cardiovascular Disease Metabolic Abnormalities Inflammatory Diseases 96-well Plate Controls Samples Replicates Reagents Pooled Control Sample Cryo-preserve HDL-C Value
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