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Immunology and Infection

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JoVE Journal Immunology and Infection
Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein
 

Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein

Article DOI: 10.3791/56325-v • 07:29 min • October 12th, 2017
October 12th, 2017 •
1, 1, 2,3, 2, 1,4, 2,4, 1
1University of California, Los Angeles, 2Centre for Biomedical Research, Burnet Institute, 3School of Health and Biomedical Sciences, RMIT University, 4Department of Infectious Diseases, Monash University

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Summary
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We describe here a fluorometric cell-free biochemical assay for determination of HDL-lipid peroxidation. This rapid and reproducible assay can be used to determine HDL function in large scale studies and can contribute to our understanding of HDL function in human disease.

Tags

Cell-free Biochemical Assay Lipid Peroxidation High Density Lipoprotein HDL Function Cardiovascular Disease Metabolic Abnormalities Inflammatory Diseases 96-well Plate Controls Samples Replicates Reagents Pooled Control Sample Cryo-preserve HDL-C Value
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