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Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein
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JoVE Journal 免疫学と感染
Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein

Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein

DOI:
10.3791/56325-v

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07:29 min

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October 12, 2017

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, , , , , ,
1University of California, Los Angeles, 2Centre for Biomedical Research,Burnet Institute, 3School of Health and Biomedical Sciences,RMIT University, 4Department of Infectious Diseases,Monash University

章

  • 00:05標題
  • 00:58Day 1: Preparation of Controls and Laboratory-specific Quality Controls
  • 02:10Day 1: Separation of HDL Cholesterol using HDL Precipitation
  • 03:07Day 2: Fluorochrome Assay
  • 05:17Results: Cell-free Biochemical Fluorometric Assay Results
  • 06:32Conclusion

概要

自動翻訳

自動翻訳

We describe here a fluorometric cell-free biochemical assay for determination of HDL-lipid peroxidation. This rapid and reproducible assay can be used to determine HDL function in large scale studies and can contribute to our understanding of HDL function in human disease.

タグ

Cell-freeBiochemicalFluorometricEnzymatic AssayHigh-throughputLipid PeroxidationHigh Density LipoproteinHDL FunctionCardiovascular DiseaseMetabolic AbnormalitiesInflammatory DiseasesHDL-CHDLoxPooled Quality ControlHDL Cholesterol Precipitating ReagentHRPFluorochrome ReagentDMSO

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