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Isolation and Characterization of Primary Rat Valve Interstitial Cells: A New Model to Study Aortic Valve Calcification
JoVE Journal
発生生物学
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JoVE Journal 発生生物学
Isolation and Characterization of Primary Rat Valve Interstitial Cells: A New Model to Study Aortic Valve Calcification

Isolation and Characterization of Primary Rat Valve Interstitial Cells: A New Model to Study Aortic Valve Calcification

9,486 Views

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08:55 min

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November 20, 2017

DOI:

10.3791/56126-v

DOI:
10.3791/56126-v

•

08:55 min
November 20, 2017

•

3 Views
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1Developmental Biology, The Roslin Institute and R(D)SVS,University of Edinburgh, 2Guangzhou Institute of Cardiovascular Disease, The Second Affiliated Hospital, School of Basic Medical Sciences,Guangzhou Medical University, 3Clinical Sciences and R(D)SVS,University of Edinburgh

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This protocol describes the isolation, culture, and calcification of rat-derived valve interstitial cells, a highly physiological in vitro model of calcific aortic valve disease (CAVD). Exploitation of this rat model facilitates CAVD research in exploring the cell and molecular mechanisms that underlie this complex pathological process.

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Lin, C., Zhu, D., Markby, G., Corcoran, B. M., Farquharson, C., Macrae, V. E. Isolation and Characterization of Primary Rat Valve Interstitial Cells: A New Model to Study Aortic Valve Calcification. J. Vis. Exp. (129), e56126, doi:10.3791/56126 (2017).

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