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Generation av infödda kromatin Immunoprecipitation sekvensering bibliotek för Nucleosome densitet analys

JoVE Journal
Genética

This content is Free Access.

JoVE Journal Genética

Generation of Native Chromatin Immunoprecipitation Sequencing Libraries for Nucleosome Density Analysis

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Generation av infödda kromatin Immunoprecipitation sekvensering bibliotek för Nucleosome densitet analys

21,530 Views

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10:05 min

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December 12, 2017

DOI:

10.3791/56085-v

DOI:

10.3791/56085-v

•

10:05 min

December 12, 2017

•

4 Views

Alireza Lorzadeh, Rodrigo Lopez Gutierrez, Linda Jackson, Michelle Moksa, Martin Hirst²

¹Department of Microbiology and Immunology, Michael Smith Laboratories Centre for High-Throughput Biology,University of British Columbia, ²Canada’s Michael Smith Genome Science Center,BC Cancer Agency

Tags

Native Chromatin Immunoprecipitation Nucleosome Density Analysis Epigenetics Epigenomic Features Chromatin Signatures MNase Cell Preparation Lysis Buffer MNase Digestion EDTA

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