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Generation of Native Chromatin Immunoprecipitation Sequencing Libraries for Nucleosome Density Analysis
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Generation of Native Chromatin Immunoprecipitation Sequencing Libraries for Nucleosome Density Analysis

Generation of Native Chromatin Immunoprecipitation Sequencing Libraries for Nucleosome Density Analysis

21,671 Views

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10:05 min

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December 12, 2017

DOI:

10.3791/56085-v

DOI:
10.3791/56085-v

•

10:05 min
December 12, 2017

•

13 Views
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1Department of Microbiology and Immunology, Michael Smith Laboratories Centre for High-Throughput Biology,University of British Columbia, 2Canada’s Michael Smith Genome Science Center,BC Cancer Agency

概要

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We present a modified native chromatin immunoprecipitation sequencing (ChIP-seq) methodology for the generation of sequence datasets suitable for a nucleosome density ChIP-seq analytical framework integrating micrococcal nuclease (MNase) accessibility with histone modification measurements.

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Cite this Article

Lorzadeh, A., Lopez Gutierrez, R., Jackson, L., Moksa, M., Hirst, M. Generation of Native Chromatin Immunoprecipitation Sequencing Libraries for Nucleosome Density Analysis. J. Vis. Exp. (130), e56085, doi:10.3791/56085 (2017).

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