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Generation of Native Chromatin Immunoprecipitation Sequencing Libraries for Nucleosome Density Analysis
Kapitel
- 00:05Titel
- 00:51Cell Lysis and Chromatin Digestion for Nucleosome Density ChIP-se
- 03:21Immunprecipitation Reaction for Nucleosome Density ChIP-seq
- 04:33Washes and Elution for Nucleosome Density ChIP-seq
- 07:31Results: Evaluation of Nucleosome Density ChIP-seq Libraries
- 08:50Conclusion
We present a modified native chromatin immunoprecipitation sequencing (ChIP-seq) methodology for the generation of sequence datasets suitable for a nucleosome density ChIP-seq analytical framework integrating micrococcal nuclease (MNase) accessibility with histone modification measurements.
