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Generation of Native Chromatin Immunoprecipitation Sequencing Libraries for Nucleosome Density Analysis
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Genética
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JoVE Journal Genética
Generation of Native Chromatin Immunoprecipitation Sequencing Libraries for Nucleosome Density Analysis

Generation of Native Chromatin Immunoprecipitation Sequencing Libraries for Nucleosome Density Analysis

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10:05 min

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December 12, 2017

DOI:

10.3791/56085-v

DOI:
10.3791/56085-v

•

10:05 min
December 12, 2017

•

13 Views
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1Department of Microbiology and Immunology, Michael Smith Laboratories Centre for High-Throughput Biology,University of British Columbia, 2Canada’s Michael Smith Genome Science Center,BC Cancer Agency

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We present a modified native chromatin immunoprecipitation sequencing (ChIP-seq) methodology for the generation of sequence datasets suitable for a nucleosome density ChIP-seq analytical framework integrating micrococcal nuclease (MNase) accessibility with histone modification measurements.

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Lorzadeh, A., Lopez Gutierrez, R., Jackson, L., Moksa, M., Hirst, M. Generation of Native Chromatin Immunoprecipitation Sequencing Libraries for Nucleosome Density Analysis. J. Vis. Exp. (130), e56085, doi:10.3791/56085 (2017).

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